EVALUATION OF GENETIC VARIABILITY OF FRESHWATER PRAWN COLLECTED FROM MAKASSAR-SULAWESI, PANGKALANBUNKALIMANTAN, JAMBI-SUMATRA, SUKABUMI-JAVA, AND GIMacro USING mtDNA CO-I MARKERS

Authors

  • Estu Nugroho Research Institute for Freshwater Aquaculture, Bogor
  • Mulyasari Mulyasari Research Institute for Freshwater Aquaculture, Bogor
  • Anang Hari Kristanto Research Institute for Freshwater Aquaculture, Bogor
  • Fauzan Ali Indonesian Science Institute, Jakarta
  • Gunawan Gunawan Indonesian Science Institute, Jakarta

DOI:

https://doi.org/10.15578/iaj.3.1.2008.23-28

Keywords:

genetic variability, freshwater prawn, mtDNA CO-I

Abstract

The objective of this research is to evaluate the genetic variability of freshwater prawn, Macrobrachium rosenbergii. The genetic variability of freshwater prawn collected from Makassar-Sulawesi, Pangkalanbun-Kalimantan, Jambi-Sumatra, Sukabumi-Java, and GIMacro strain was examined using polymorphism of the mitochondria DNA (mtDNA) markers. Twelve composite haplotypes were detected following digestion of CO1 sequences with four endonucleases: Hae III, Rsa I, Mbo I, and Taq I. The average haplotype diversity was 0.217. Significant genetic difference was observed among freshwater prawn populations, especially among Makassar-Sulawesi population and others. Makassar-Sulawesi strain has future prospect for genetic resources in breeding program.

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Published

2009-06-30

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Articles