DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH

Authors

  • Huria Marnis Research Institute for Fish Breeding, Sukamandi
  • Bambang Iswanto Research Institute for Fish Breeding, Sukamandi
  • Rommy Suprapto Research Institute for Fish Breeding, Sukamandi
  • Imron Imron Research Institute for Fish Breeding, Sukamandi
  • Raden Roro Sri Pudji Sinarni Dewi Research Institute for Fish Breeding, Sukamandi

DOI:

https://doi.org/10.15578/iaj.9.2.2014.89-95

Keywords:

African catfish, transgenic, growth hormone, transmission, gene expression

Abstract

In previous study, the fast growth transgenic founder of African catfish was produced harboring a growth hormone (GH) gene construct containing a stripped catfish growth hormone (PhGH) cDNA. This study was conducted to investigate transgene (PhGH) transmission and expression in F-1 transgenic African catfish. The transgenic founders (female) were crossed with non-transgenic (male) to produce heterozygous F-1 progeny. PhGH gene was detected in the embryo, larvae, and seed of the transgenic F-1 using PCR method. Expression levels of transgene in embryo and larvae were analyzed using real-time quantitative PCR (qPCR) method. The transgene was detected in embryo, larvae and seed of F-1 transgenic African catfish. Founder could transmit PhGH gene to transgenic F-1 lines in ranged 36% to 48%. Expression level of Phgh gene in embryo was higher than that of the larvae; whereas in the embryo was 1.5 x 105 - 5.2 x 105 copies or 0.49-9.82 fold, while in the larvae was 1.1 x 105 - 2.5 x 105
copies or 0.19-5.80 fold. 

Downloads

Published

2014-12-31

Issue

Vol. 9 No. 2 (2014): (December 2014)

Section

Articles

License

Creative Commons License
Indonesian Aquaculture Journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright Transfer Terms

The author in submitting online understands that if the manuscript is accepted for publication, the copyright of the manuscript will be given to the Indonesian Aquaculture Journal as the publisher of the journal.

Copyright includes the exclusive right to reproduce and distribute articles in all forms of media, including reprints, photographs, microfilm and other similar reproductions, as well as their translations.